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Literature summary for 7.1.1.6 extracted from

  • Kolling, D.J.; Brunzelle, J.S.; Lhee, S.; Crofts, A.R.; Nair, S.K.
    Atomic resolution structures of Rieske iron-sulfur protein: role of hydrogen bonds in tuning the redox potential of iron-sulfur clusters (2007), Structure, 15, 29-38.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.1.1.6

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method using 1.5 M [NH4]2SO4, 100 mM sodium acetate at pH 4.6 Cereibacter sphaeroides

Protein Variants

Protein Variants Comment Organism
S154A results in a 97 mV decrease in the midpoint potential relative to the wild-type and a 5fold decrease in sensitivity to inhibition by stigmatellin Cereibacter sphaeroides
S154C results in a 17 mV increase in the midpoint potential relative to the wild-type Cereibacter sphaeroides
S154T results in a 72 mV increase in the midpoint potential relative to the wild-type Cereibacter sphaeroides
Y156F results in a 45 mV decrease in the midpoint potential relative to the wild-type Cereibacter sphaeroides
Y156W results in a decrease in the midpoint potential by 100 mV and substantial changes in the EPR spectra relative to the wild-type enzyme Cereibacter sphaeroides

Inhibitors

Inhibitors Comment Organism Structure
Stigmatellin
-
 Cereibacter sphaeroides

Metals/Ions

Metals/Ions Comment Organism Structure
2Fe-2S
-
 Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-affinity resin chromatography and hydrophobic interaction chromatography Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
plastoquinol + plastocyanin
-
 Cereibacter sphaeroides plastoquinone + reduced plastocyanin
-
 ?

Synonyms

Synonyms Comment Organism
Rieske iron-sulfur protein
-
 Cereibacter sphaeroides
Rieske ISP
-
 Cereibacter sphaeroides
Rieske [2Fe-2S] protein
-
 Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
heme
-
 Cereibacter sphaeroides